artificial cerebrospinal fluid中文什么意思

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in vitro injury models of brain slice ( ogd and nmda insult ) and primary neuronal cultures ( nmda insult ) oxygen / glucose deprivation ( ogd )-induced injury of rat hippocampal slice in vitro the rat hippocampal slices prepared were allowed to recover in the normal artificial cerebrospinal fluid ( acsf ) bubbled with gas mixture of 95 % o2 + 5 % co2 for 1 h, then they were thansfered to glucose-free nacsf which was bubbled with gas mixture of 95 % n2 + 5 % co2 . after treatment with ogd, the slices were placed into 2 % ttc solution in dark and incubated at 37 * cfor 1h . the slices were weighted and a 50 : 50 mixture of ethanol / dimethyl sulfoxide was then added to extract the formazan in dark for 24 h
离体脑片损伤模型（ogd和nmda）及原代培养神经元nmda损伤大鼠离体海马脑片缺氧缺糖（ogd）损伤大鼠离体海马脑片制备后，在通氧混合气的正常脑脊液（nacsf）中恢复60min，然后移入通氮混合气的无糖脑脊液（吵化sf）中缺氧缺糖，取出脑片与2ttc避光37’c温浴60dll，染色后根据每克湿重加入20ml抽提液（乙醇：二甲亚矾一50：50），在密闭容器内避光置24h，测量前摇匀后取200pi至96孔板，在490urn波长，酶标仪测定各孔od值。

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